Effect of heterologous combination on competitive nephelometric immunoassay. II. Bridge-heterologous combination for ethosuximide immunoassay.

Competitive enzyme immunoassay for apolipoprotein A-II.

A competitive enzyme immunoassay for apolipoprotein A-II was developed. Microtitre plates were used as a solid phase and coated with anti-apolipoprotein A-II antibodies. Purified apolipoprotein A-II, labelled with horseradish peroxidase was used as competing ligand. The assay was examined with respect to the optimal amounts of specific anti-apolipoprotein A-II antibodies and apolipoprotein A-II...

Nephelometric immunoassay with shell/core particles

Nephelometry has become a very reliable and convenient method for immunoassays. Recent innovations in polymer chemistry have led to the design of highly sensitive and reproducible immunoassays. Key advances include the preparation and use of particles with polystyrene cores covered by thin shells with chemically reactive groups. The shells allow a covalent binding of the immunochemicals of inte...

Study of Calcification of Teeth by Combination of Heterologous Tissues

We focused attention of the calcification ability of coral cells. A section of coral was transplanted to the cavity of a tooth and observed microscopically for calcification after 7 days (LI group) and 14 days (LII group) of artificial irradiation. Observation after 7 days (NI group) and 14 days (NII group) without artificial irradiation was used as a control. Calcification was observed in the ...

Multisite monoclonal immunoassay for dengue viruses: detection of viraemic human sera and interference by heterologous antibody.

A monoclonal radioimmunoassay (RIA) was developed for detection of dengue virus in infected cell culture fluids and blood samples from dengue patients. Antibodies used to construct the RIA were selected on the basis of high binding avidity, the demonstration of synergism in competitive binding assays and empirical trials with different antibody combinations. Optimal binding of all four dengue v...

Multiplexing-Capable Immunobiosensor Based on Competitive Immunoassay